cd45 1 apc cy7 tonbo bioscience Search Results


anti cd45 1 apc cy7  (Cytek Biosciences)
96
Cytek Biosciences anti cd45 1 apc cy7
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cd45 1 pe cy7 tonbo biosciences a20  (Cytek Biosciences)
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α-mouse cd45.1 pe-cy7  (Cytek Biosciences)
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KEY RESOURCES TABLE
α Mouse Cd45.1 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd45.1-pe/cy7 antibody  (Thermo Fisher)
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KEY RESOURCES TABLE
Cd45.1 Pe/Cy7 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apc cy7 anti mouse cd45 1  (Cytek Biosciences)
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KEY RESOURCES TABLE
Apc Cy7 Anti Mouse Cd45 1, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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35 0453 u500  (Cytek Biosciences)
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KEY RESOURCES TABLE
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anti cd45 1  (Cytek Biosciences)
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Cytek Biosciences anti cd45 1
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Anti Cd45 1, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-cy7-cd11b  (Thermo Fisher)
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α-f4/80 (bm8) efluor 450  (Thermo Fisher)
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Thermo Fisher α-f4/80 (bm8) efluor 450
(A) Adoptive transfer and immunization scheme. (B) Representative flow plots using the T FH markers PD-1 and CXCR5 or Bcl-6 and CXCR5 respectively. Cells were pregated <t>as</t> <t>CD45.1+,</t> CD4+, MHC II-, CD19-, CD8α-, F4/80-. Numbers indicate percentages of CA30 T cells with indicated T FH markers. (C) Numbers of CA30 T cells in individual mice with the indicated T FH markers. Statistical differences were determined by a two-tailed Mann-Whitney U test. Data are representative of three independent experiments with n = 5 mice per group.
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cd45.1 a20, v450  (Becton Dickinson)
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Becton Dickinson cd45.1 a20, v450
(A) Adoptive transfer and immunization scheme. (B) Representative flow plots using the T FH markers PD-1 and CXCR5 or Bcl-6 and CXCR5 respectively. Cells were pregated <t>as</t> <t>CD45.1+,</t> CD4+, MHC II-, CD19-, CD8α-, F4/80-. Numbers indicate percentages of CA30 T cells with indicated T FH markers. (C) Numbers of CA30 T cells in individual mice with the indicated T FH markers. Statistical differences were determined by a two-tailed Mann-Whitney U test. Data are representative of three independent experiments with n = 5 mice per group.
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ghost dye red 780  (Cytek Biosciences)
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Cytek Biosciences ghost dye red 780
(A) Adoptive transfer and immunization scheme. (B) Representative flow plots using the T FH markers PD-1 and CXCR5 or Bcl-6 and CXCR5 respectively. Cells were pregated <t>as</t> <t>CD45.1+,</t> CD4+, MHC II-, CD19-, CD8α-, F4/80-. Numbers indicate percentages of CA30 T cells with indicated T FH markers. (C) Numbers of CA30 T cells in individual mice with the indicated T FH markers. Statistical differences were determined by a two-tailed Mann-Whitney U test. Data are representative of three independent experiments with n = 5 mice per group.
Ghost Dye Red 780, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe conjugated cd11b  (Cytek Biosciences)
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Cytek Biosciences pe conjugated cd11b
(A) Adoptive transfer and immunization scheme. (B) Representative flow plots using the T FH markers PD-1 and CXCR5 or Bcl-6 and CXCR5 respectively. Cells were pregated <t>as</t> <t>CD45.1+,</t> CD4+, MHC II-, CD19-, CD8α-, F4/80-. Numbers indicate percentages of CA30 T cells with indicated T FH markers. (C) Numbers of CA30 T cells in individual mice with the indicated T FH markers. Statistical differences were determined by a two-tailed Mann-Whitney U test. Data are representative of three independent experiments with n = 5 mice per group.
Pe Conjugated Cd11b, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell stem cell

Article Title: Restricted Hematopoietic Progenitors and Erythropoiesis Require SCF from Leptin Receptor+ Niche Cells in the Bone Marrow

doi: 10.1016/j.stem.2018.11.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: α-mouse CD45.1 PE-Cy7 , Tonbo Biosciences , A20; RRID:AB_2621850.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

(A) Adoptive transfer and immunization scheme. (B) Representative flow plots using the T FH markers PD-1 and CXCR5 or Bcl-6 and CXCR5 respectively. Cells were pregated as CD45.1+, CD4+, MHC II-, CD19-, CD8α-, F4/80-. Numbers indicate percentages of CA30 T cells with indicated T FH markers. (C) Numbers of CA30 T cells in individual mice with the indicated T FH markers. Statistical differences were determined by a two-tailed Mann-Whitney U test. Data are representative of three independent experiments with n = 5 mice per group.

Journal: PLoS ONE

Article Title: Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes

doi: 10.1371/journal.pone.0170556

Figure Lengend Snippet: (A) Adoptive transfer and immunization scheme. (B) Representative flow plots using the T FH markers PD-1 and CXCR5 or Bcl-6 and CXCR5 respectively. Cells were pregated as CD45.1+, CD4+, MHC II-, CD19-, CD8α-, F4/80-. Numbers indicate percentages of CA30 T cells with indicated T FH markers. (C) Numbers of CA30 T cells in individual mice with the indicated T FH markers. Statistical differences were determined by a two-tailed Mann-Whitney U test. Data are representative of three independent experiments with n = 5 mice per group.

Article Snippet: The following antibodies were used: from eBioscience: α-CD8α (53–6.7) eFluor 450, α-CD45.1 (A20) APC, α-F4/80 (BM8) eFluor 450, α-CD19 (eBio1D3) eFluor 450, α-Vβ8.1/Vβ8.2 (KJ16-133) FITC, α-MHCII I-A/I-E (M5/114.15.2) eFluor 450, Rat IgG2a/κ isotype control (eBR2a) PE, α-BCL6 (BCL-DWN) PE, and α-CXCR5 (SPRCL5) Biotin; Biolegend: α-CD4 (GK1.5) APC-Cy7 and APC, α-B220/CD45R (RA3-6B2) FITC, PE, APC, APC-Cy7, eFluor 450, and α-PD-1 (RMP1-30) PE; Thermo Fisher: streptavidin-APC; Tonbo Biosciences: α-CD45.1 (A20) APC.

Techniques: Adoptive Transfer Assay, Two Tailed Test, MANN-WHITNEY

(A) Adoptive transfer scheme. (B) Representative FACS plots of CFSE and CD45.1 staining in the CD4+, MHC II-, CD19-, CD8α-, F4/80- gate and representative CFSE histograms for CD4+ CD45.1+ (CA30) cells. (C) Graph of percentage or total numbers (D) of CD4+ CD45.1+ (CA30) cells with SEM in each cell cycle division gate as defined by the FlowJo CFSE proliferation algorithm. Data presented are from a single experiment with mice treated with deaggregated (n = 7), immune complex (n = 7), or heat aggregated (n = 4) mAb 36–71. Statistical differences were determined by a two-tailed Mann-Whitney U test (* = p<0.05, ** = p<0.005). Data are representative of 3 independent experiments using n ≥ 4 mice per group.

Journal: PLoS ONE

Article Title: Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes

doi: 10.1371/journal.pone.0170556

Figure Lengend Snippet: (A) Adoptive transfer scheme. (B) Representative FACS plots of CFSE and CD45.1 staining in the CD4+, MHC II-, CD19-, CD8α-, F4/80- gate and representative CFSE histograms for CD4+ CD45.1+ (CA30) cells. (C) Graph of percentage or total numbers (D) of CD4+ CD45.1+ (CA30) cells with SEM in each cell cycle division gate as defined by the FlowJo CFSE proliferation algorithm. Data presented are from a single experiment with mice treated with deaggregated (n = 7), immune complex (n = 7), or heat aggregated (n = 4) mAb 36–71. Statistical differences were determined by a two-tailed Mann-Whitney U test (* = p<0.05, ** = p<0.005). Data are representative of 3 independent experiments using n ≥ 4 mice per group.

Article Snippet: The following antibodies were used: from eBioscience: α-CD8α (53–6.7) eFluor 450, α-CD45.1 (A20) APC, α-F4/80 (BM8) eFluor 450, α-CD19 (eBio1D3) eFluor 450, α-Vβ8.1/Vβ8.2 (KJ16-133) FITC, α-MHCII I-A/I-E (M5/114.15.2) eFluor 450, Rat IgG2a/κ isotype control (eBR2a) PE, α-BCL6 (BCL-DWN) PE, and α-CXCR5 (SPRCL5) Biotin; Biolegend: α-CD4 (GK1.5) APC-Cy7 and APC, α-B220/CD45R (RA3-6B2) FITC, PE, APC, APC-Cy7, eFluor 450, and α-PD-1 (RMP1-30) PE; Thermo Fisher: streptavidin-APC; Tonbo Biosciences: α-CD45.1 (A20) APC.

Techniques: Adoptive Transfer Assay, Staining, Two Tailed Test, MANN-WHITNEY

(A) Adoptive transfer and immunization protocol using deaggregated intact mAb 36–71 (100 μg) or the molar equivalent of its Fab (66 μg) or F(ab’) 2 (73 μg) fragments, all deaggregated. (B) Representative CFSE histograms for CD4+ CD45.1+ (CA30) cells pregated in the CD4+, MHC II-, CD19-, CD8α-, F4/80- gate. (C) Graph of percentage of CD4+ CD45.1+ (CA30) cells with SEM in each cell cycle division gate as defined by the FlowJo CFSE proliferation algorithm. Data presented are from a single experiment with mice treated with deaggregated (n = 5), Fab (n = 5), or F(ab’) 2 (n = 5) mAb 36–71. Statistical differences were determined by a two-tailed Mann-Whitney U test (* = p<0.05, ** = p<0.005). Data are representative of 2 independent experiments using n > 4 mice per group.

Journal: PLoS ONE

Article Title: Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes

doi: 10.1371/journal.pone.0170556

Figure Lengend Snippet: (A) Adoptive transfer and immunization protocol using deaggregated intact mAb 36–71 (100 μg) or the molar equivalent of its Fab (66 μg) or F(ab’) 2 (73 μg) fragments, all deaggregated. (B) Representative CFSE histograms for CD4+ CD45.1+ (CA30) cells pregated in the CD4+, MHC II-, CD19-, CD8α-, F4/80- gate. (C) Graph of percentage of CD4+ CD45.1+ (CA30) cells with SEM in each cell cycle division gate as defined by the FlowJo CFSE proliferation algorithm. Data presented are from a single experiment with mice treated with deaggregated (n = 5), Fab (n = 5), or F(ab’) 2 (n = 5) mAb 36–71. Statistical differences were determined by a two-tailed Mann-Whitney U test (* = p<0.05, ** = p<0.005). Data are representative of 2 independent experiments using n > 4 mice per group.

Article Snippet: The following antibodies were used: from eBioscience: α-CD8α (53–6.7) eFluor 450, α-CD45.1 (A20) APC, α-F4/80 (BM8) eFluor 450, α-CD19 (eBio1D3) eFluor 450, α-Vβ8.1/Vβ8.2 (KJ16-133) FITC, α-MHCII I-A/I-E (M5/114.15.2) eFluor 450, Rat IgG2a/κ isotype control (eBR2a) PE, α-BCL6 (BCL-DWN) PE, and α-CXCR5 (SPRCL5) Biotin; Biolegend: α-CD4 (GK1.5) APC-Cy7 and APC, α-B220/CD45R (RA3-6B2) FITC, PE, APC, APC-Cy7, eFluor 450, and α-PD-1 (RMP1-30) PE; Thermo Fisher: streptavidin-APC; Tonbo Biosciences: α-CD45.1 (A20) APC.

Techniques: Adoptive Transfer Assay, Two Tailed Test, MANN-WHITNEY